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Thermo Fisher chaps
Chaps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chaps/CHAPS/pm42176793-62-21-22
Average 96 stars, based on 1 article reviews

chaps - by Bioz Stars, 2026-10

96/100 stars

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Extraction:

Article Title: Granular Extracellular Matrix (gECM) Hydrogels Enable Distinct Composition and Mechanics Across Tissue Types for Translation
Article Snippet: .. For three-fraction extraction, 3 mg of lyophilized material from each bone, skin, liver, and kidney sample was combined with 100 mg of 1 mm glass beads (Next Advance #GB10) and homogenized in decellularization buffer (50 mM Tris-HCl (pH 7.4), 0.25% CHAPS, 25 mM EDTA, 3 M NaCl, 1X HaltTM Protease Inhibitor Cocktail (Thermo Scientific #78438)) at power 8 for 3 min (Bullet Blender, Model BBX24, Next Advance, Inc.). .. For three-fraction extraction, 3 mg of lyophilized material from each bone, skin, liver, and kidney sample was combined with 100 mg of 1 mm glass beads (Next Advance #GB10) and homogenized in decellularization buffer (50 mM Tris-HCl (pH 7.4), 0.25% CHAPS, 25 mM EDTA, 3 M NaCl, 1X Halt Protease Inhibitor Cocktail (Thermo Scientific #78438)) at power 8 for 3 min (Bullet Blender, Model BBX24, Next Advance, Inc.).

Protease Inhibitor:

Article Title: Nucleic acids and nucleic acid analogs for treating, preventing, and disrupting pathological polynucleotide-binding protein inclusions
Article Snippet: .. The resulting supernatant was discarded and pellets were resuspended in urea buffer (30 mM Tris pH 8.5 (Sigma-Aldrich), 7 M urea (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich), 4% CHAPS (Thermo Fisher Scientific), protease inhibitor cocktail (Sigma-Aldrich), 1 mM PMSF (Thermo Fisher Scientific), phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich)). ..

Lysis:

Article Title: Kinase-independent signaling by PIM1 promotes drug resistance by increasing mitophagy and reducing oxidative stress.
Article Snippet: .. Cells were lysed with 500 mL of lysis buffer containing 40 mM HEPES (pH 7.6), 120 mM NaCl (Fisher, #BP358-212), 0.3% CHAPS (Thermo Scientific, #28300), 10 mM NaF (Fisher, #S299), 10 mM bglycerol phosphate, 1 mM EDTA (pH 8.0) (Fisher, #O2793-500), 2 mM sodium orthovanadate, 17 mg/mL aprotinin,10 mg/mL leupeptin, and 1 mM PMSF. ..

Article Title: Vesicular Rps6 Released by Astrocytes in an Experimental Model of AD Regulates Local Translation and Enhances Synaptic Integrity in Neurones
Article Snippet: .. Proteins were extracted using a lysis buffer containing 7 M urea (Fisher Chemical), 2 M thiourea (Alfa Aesar), 4% CHAPS (Acros Organics, Geel, Belgium) and 5 mM DTT in water. .. Peptides were desalted using C18 tips (Millipore, Burlington, MA, USA) and analyzed by LC‐MS/MS in a timsTOF Pro with PASEF spectrometer (Bruker Daltonics, Billerica, MA, USA) connected to an Evosep ONE chromatograph (Evosep, Odense, Denmark).

Article Title: Estrogen protects against ethanol-induced astrocytic response in dorsal hippocampus but not ethanol-induced liver inflammation in ovariectomized mice.
Article Snippet: Sex has been shown to play a dynamic role in the responses to alcohol, with women often being cited as having more detrimental consequences from alcohol use.. The current increasing rates of alcohol drinking by women, including women over 50, highlights the importance of studying the participation of sex hormones in the effects of alcohol misuse.. Alcohol is known to produce both central and peripheric inflammation, but the interaction between alcohol and estradiol is poorly understood.

Article Title: Vesicular Rps6 Released by Astrocytes in an Experimental Model of AD Regulates Local Translation and Enhances Synaptic Integrity in Neurones.
Article Snippet: .. Proteins were extracted using a lysis buffer containing 7 M urea (Fisher Chemical), 2 M thiourea (Alfa Aesar), 4% CHAPS (Acros Organics, Geel, Belgium) and 5 mM DTT in water. .. Peptides were desalted using C18 tips (Millipore, Burlington, MA, USA) and analyzed by LC-MS/MS in a timsTOF Pro with PASEF spectrometer (Bruker Daltonics, Billerica, MA, USA) connected to an Evosep ONE chromatograph (Evosep, Odense, Denmark).

Concentration Assay:

Article Title: Pharmacological PIK3C2B inhibition rescues XLMTM phenotype in mouse models and identifies molecular markers of disease.
Article Snippet: 384 PIK3C2B biochemical assay 385 The potency of compounds against the in vitro enzymatic activity of human PIK3C2B was determined by 386 ADP-Glo format (Promega, USA). .. The lipid kinase reaction was performed in 10 mM MgCl2, 100 mM 387 NaCl, 1 mM EGTA, 0.03% CHAPS, 2 mM DTT, and 50 mM HEPES, at pH 7.5.. To initiate the enzyme 388 reaction, 120 nM of human PIK3C2B enzyme (Cat# PV3574l, Thermo Scientific, USA) and 2 mM ATP 389 (Sigma Aldrich, USA) were preincubated with compounds at 2x final concentration for 10 minutes, 390 followed by the addition of an equal volume of 300 nM lipid substrate phosphatidylinositol diC8 (Cat# P-391 0008, Echelon Biosciences, USA) to a final concentration of 60 nM PIK3C2B, 1 mM ATP, and 150 nM diC8.. 392 ADP generated during the kinase reaction was resynthesized to ATP, which was determined as a 393 luminescence signal in a coupled luciferase/luciferin reaction with an EnVision Multimode Plate reader 394 (Revvity, USA). ..

Generated:

Luciferase:

other:

Article Title: Antibodies that specifically bind peptides associated with the primary immunodeficiencies: Wiskott-Aldrich syndrome and x-linked agammaglobulinemia
Article Snippet: Briefly, antibody beads were collected using magnetic pulldown, excess PBS was discarded, and 300 μL of freshly prepared 20 mM DMP (dimethyl pimelimidate dihydrochloride, no. D8388, Sigma Aldrich, St. Louis, MO) in 200 mM triethanolamine, pH 8.5 (no. T58300, Sigma Aldrich, St. Louis, MO) was added.

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